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t47d  (ATCC)


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    Structured Review

    ATCC t47d
    Expression of Notch1 and Notch2 in breast cancer cells and interaction with SNOs. a Human MDA-MB231, b human ZR75D, c mouse 4T1, d human <t>T47D</t> and e human BT747 BrCa cell lines were analysed by flow cytometry for their expression of Notch1 and Notch2. f Quantification of human MDA-MB231 GFP + BrCa cells (MDA GFP ) MACS-sorted for low or high expression of Notch1 after 72 h of culture on SNO and NON-SNO monolayers. g Quantification of human MDA-MB231 GFP + BrCa cells (MDA GFP ) MACS-sorted for low or high expression of Notch2 after 72 h of culture on SNO and NON-SNO monolayers. h Quantification of human T47D BrCa cells labelled with the PKH26 membrane dye, MACS-sorted for low or high expression of Notch1 after 72 h of culture on SNO and NON-SNO monolayers. Results are ( a – e ) representative or ( f – h ) the mean ± SD of 3 independent experiments. Statistics: unpaired t -test
    T47d, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 6751 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t47d/product/ATCC
    Average 99 stars, based on 6751 article reviews
    t47d - by Bioz Stars, 2026-06
    99/100 stars

    Images

    1) Product Images from "The endosteal niche regulates breast cancer cell dormancy in bone: identification of new molecular determinants"

    Article Title: The endosteal niche regulates breast cancer cell dormancy in bone: identification of new molecular determinants

    Journal: Bone Research

    doi: 10.1038/s41413-026-00535-3

    Expression of Notch1 and Notch2 in breast cancer cells and interaction with SNOs. a Human MDA-MB231, b human ZR75D, c mouse 4T1, d human T47D and e human BT747 BrCa cell lines were analysed by flow cytometry for their expression of Notch1 and Notch2. f Quantification of human MDA-MB231 GFP + BrCa cells (MDA GFP ) MACS-sorted for low or high expression of Notch1 after 72 h of culture on SNO and NON-SNO monolayers. g Quantification of human MDA-MB231 GFP + BrCa cells (MDA GFP ) MACS-sorted for low or high expression of Notch2 after 72 h of culture on SNO and NON-SNO monolayers. h Quantification of human T47D BrCa cells labelled with the PKH26 membrane dye, MACS-sorted for low or high expression of Notch1 after 72 h of culture on SNO and NON-SNO monolayers. Results are ( a – e ) representative or ( f – h ) the mean ± SD of 3 independent experiments. Statistics: unpaired t -test
    Figure Legend Snippet: Expression of Notch1 and Notch2 in breast cancer cells and interaction with SNOs. a Human MDA-MB231, b human ZR75D, c mouse 4T1, d human T47D and e human BT747 BrCa cell lines were analysed by flow cytometry for their expression of Notch1 and Notch2. f Quantification of human MDA-MB231 GFP + BrCa cells (MDA GFP ) MACS-sorted for low or high expression of Notch1 after 72 h of culture on SNO and NON-SNO monolayers. g Quantification of human MDA-MB231 GFP + BrCa cells (MDA GFP ) MACS-sorted for low or high expression of Notch2 after 72 h of culture on SNO and NON-SNO monolayers. h Quantification of human T47D BrCa cells labelled with the PKH26 membrane dye, MACS-sorted for low or high expression of Notch1 after 72 h of culture on SNO and NON-SNO monolayers. Results are ( a – e ) representative or ( f – h ) the mean ± SD of 3 independent experiments. Statistics: unpaired t -test

    Techniques Used: Expressing, Flow Cytometry, Membrane



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    Expression of Notch1 and Notch2 in breast cancer cells and interaction with SNOs. a Human MDA-MB231, b human ZR75D, c mouse 4T1, d human <t>T47D</t> and e human BT747 BrCa cell lines were analysed by flow cytometry for their expression of Notch1 and Notch2. f Quantification of human MDA-MB231 GFP + BrCa cells (MDA GFP ) MACS-sorted for low or high expression of Notch1 after 72 h of culture on SNO and NON-SNO monolayers. g Quantification of human MDA-MB231 GFP + BrCa cells (MDA GFP ) MACS-sorted for low or high expression of Notch2 after 72 h of culture on SNO and NON-SNO monolayers. h Quantification of human T47D BrCa cells labelled with the PKH26 membrane dye, MACS-sorted for low or high expression of Notch1 after 72 h of culture on SNO and NON-SNO monolayers. Results are ( a – e ) representative or ( f – h ) the mean ± SD of 3 independent experiments. Statistics: unpaired t -test
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    Expression of Notch1 and Notch2 in breast cancer cells and interaction with SNOs. a Human MDA-MB231, b human ZR75D, c mouse 4T1, d human <t>T47D</t> and e human BT747 BrCa cell lines were analysed by flow cytometry for their expression of Notch1 and Notch2. f Quantification of human MDA-MB231 GFP + BrCa cells (MDA GFP ) MACS-sorted for low or high expression of Notch1 after 72 h of culture on SNO and NON-SNO monolayers. g Quantification of human MDA-MB231 GFP + BrCa cells (MDA GFP ) MACS-sorted for low or high expression of Notch2 after 72 h of culture on SNO and NON-SNO monolayers. h Quantification of human T47D BrCa cells labelled with the PKH26 membrane dye, MACS-sorted for low or high expression of Notch1 after 72 h of culture on SNO and NON-SNO monolayers. Results are ( a – e ) representative or ( f – h ) the mean ± SD of 3 independent experiments. Statistics: unpaired t -test
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    Expression of Notch1 and Notch2 in breast cancer cells and interaction with SNOs. a Human MDA-MB231, b human ZR75D, c mouse 4T1, d human <t>T47D</t> and e human BT747 BrCa cell lines were analysed by flow cytometry for their expression of Notch1 and Notch2. f Quantification of human MDA-MB231 GFP + BrCa cells (MDA GFP ) MACS-sorted for low or high expression of Notch1 after 72 h of culture on SNO and NON-SNO monolayers. g Quantification of human MDA-MB231 GFP + BrCa cells (MDA GFP ) MACS-sorted for low or high expression of Notch2 after 72 h of culture on SNO and NON-SNO monolayers. h Quantification of human T47D BrCa cells labelled with the PKH26 membrane dye, MACS-sorted for low or high expression of Notch1 after 72 h of culture on SNO and NON-SNO monolayers. Results are ( a – e ) representative or ( f – h ) the mean ± SD of 3 independent experiments. Statistics: unpaired t -test
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    Expression of Notch1 and Notch2 in breast cancer cells and interaction with SNOs. a Human MDA-MB231, b human ZR75D, c mouse 4T1, d human <t>T47D</t> and e human BT747 BrCa cell lines were analysed by flow cytometry for their expression of Notch1 and Notch2. f Quantification of human MDA-MB231 GFP + BrCa cells (MDA GFP ) MACS-sorted for low or high expression of Notch1 after 72 h of culture on SNO and NON-SNO monolayers. g Quantification of human MDA-MB231 GFP + BrCa cells (MDA GFP ) MACS-sorted for low or high expression of Notch2 after 72 h of culture on SNO and NON-SNO monolayers. h Quantification of human T47D BrCa cells labelled with the PKH26 membrane dye, MACS-sorted for low or high expression of Notch1 after 72 h of culture on SNO and NON-SNO monolayers. Results are ( a – e ) representative or ( f – h ) the mean ± SD of 3 independent experiments. Statistics: unpaired t -test
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    Expression of Notch1 and Notch2 in breast cancer cells and interaction with SNOs. a Human MDA-MB231, b human ZR75D, c mouse 4T1, d human <t>T47D</t> and e human BT747 BrCa cell lines were analysed by flow cytometry for their expression of Notch1 and Notch2. f Quantification of human MDA-MB231 GFP + BrCa cells (MDA GFP ) MACS-sorted for low or high expression of Notch1 after 72 h of culture on SNO and NON-SNO monolayers. g Quantification of human MDA-MB231 GFP + BrCa cells (MDA GFP ) MACS-sorted for low or high expression of Notch2 after 72 h of culture on SNO and NON-SNO monolayers. h Quantification of human T47D BrCa cells labelled with the PKH26 membrane dye, MACS-sorted for low or high expression of Notch1 after 72 h of culture on SNO and NON-SNO monolayers. Results are ( a – e ) representative or ( f – h ) the mean ± SD of 3 independent experiments. Statistics: unpaired t -test
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    Image Search Results


    Expression of Notch1 and Notch2 in breast cancer cells and interaction with SNOs. a Human MDA-MB231, b human ZR75D, c mouse 4T1, d human T47D and e human BT747 BrCa cell lines were analysed by flow cytometry for their expression of Notch1 and Notch2. f Quantification of human MDA-MB231 GFP + BrCa cells (MDA GFP ) MACS-sorted for low or high expression of Notch1 after 72 h of culture on SNO and NON-SNO monolayers. g Quantification of human MDA-MB231 GFP + BrCa cells (MDA GFP ) MACS-sorted for low or high expression of Notch2 after 72 h of culture on SNO and NON-SNO monolayers. h Quantification of human T47D BrCa cells labelled with the PKH26 membrane dye, MACS-sorted for low or high expression of Notch1 after 72 h of culture on SNO and NON-SNO monolayers. Results are ( a – e ) representative or ( f – h ) the mean ± SD of 3 independent experiments. Statistics: unpaired t -test

    Journal: Bone Research

    Article Title: The endosteal niche regulates breast cancer cell dormancy in bone: identification of new molecular determinants

    doi: 10.1038/s41413-026-00535-3

    Figure Lengend Snippet: Expression of Notch1 and Notch2 in breast cancer cells and interaction with SNOs. a Human MDA-MB231, b human ZR75D, c mouse 4T1, d human T47D and e human BT747 BrCa cell lines were analysed by flow cytometry for their expression of Notch1 and Notch2. f Quantification of human MDA-MB231 GFP + BrCa cells (MDA GFP ) MACS-sorted for low or high expression of Notch1 after 72 h of culture on SNO and NON-SNO monolayers. g Quantification of human MDA-MB231 GFP + BrCa cells (MDA GFP ) MACS-sorted for low or high expression of Notch2 after 72 h of culture on SNO and NON-SNO monolayers. h Quantification of human T47D BrCa cells labelled with the PKH26 membrane dye, MACS-sorted for low or high expression of Notch1 after 72 h of culture on SNO and NON-SNO monolayers. Results are ( a – e ) representative or ( f – h ) the mean ± SD of 3 independent experiments. Statistics: unpaired t -test

    Article Snippet: The human BrCa cell lines MDA-MB231, T47D, ZR75D, BT474, and the murine BrCa cell line 4T1 were purchased from the American Type Culture Collection (ATCC) and cultured according to the supplier instruction.

    Techniques: Expressing, Flow Cytometry, Membrane